Journal: Antibiotics

Article Title: Increased Susceptibility of Mycobacterium tuberculosis to Ethionamide by Expressing PPs-Induced Rv0560c

doi: 10.3390/antibiotics11101349

Figure Lengend Snippet: Gene expression changes in PPs-treated M. tuberculosis H37Rv. ( A ) Two-dimensional gel electrophoresis (2DE) analysis for protein expression in M. tuberculosis after treatment with 1× MIC and 10× MIC PP1S at 37 °C for 6 h. ( B ) Protein spots showing significantly different expressions between PP1S treated and untreated groups were identified. Two spots whose expressions were significantly increased by PP1S were found to be Rv0560c. ( C ) As a result of confirming the gene transcription pattern through microarray under the same conditions as 2DE, the most upregulated gene by PPs was Rv0560c. Overexpression of Rv0560c by PPs was confirmed by ( D ) RT-PCR and ( E ) Western blotting using an Rv0560c-specific antibody. ( D , E ) Rv0560c gene was also upregulated by salicylate (SAL). RT-PCR results are expressed as mean ± standard deviation.

Article Snippet: 2DE experiment was performed by ProteomeTech (Seoul, Korea) as previously described [ ].

Techniques: Gene Expression, Two-Dimensional Gel Electrophoresis, Electrophoresis, Expressing, Microarray, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Standard Deviation

Journal: Journal of Proteomics

Article Title: Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization

doi: 10.1016/j.jprot.2015.12.029

Figure Lengend Snippet: Two dimensional gel electrophoresis (2DE) of protein homogenates from human tonsils no. 1. MH: 2DE of the mechanical homogenate (MH) of human tonsils (m = 200 μg). DH: 2DE of the DIVE homogenate (DH) of human tonsils (m = 200 μg). Spots identified by LC–MS/MS and described in the text, and in supplemental results are marked with a number (DH) or with an asterisk and a number (MH).

Article Snippet: 2DE gels were stained with FireSilver (Proteome Factory, Berlin, Germany).

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Liquid Chromatography with Mass Spectroscopy

Journal: Journal of Proteomics

Article Title: Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization

doi: 10.1016/j.jprot.2015.12.029

Figure Lengend Snippet: Proteins identified in selected spots of the 2DE gel (Fig. 1) of the DIVE homogenate (DH) and the mechanical homogenate (MH). X: spot was not present.

Article Snippet: 2DE gels were stained with FireSilver (Proteome Factory, Berlin, Germany).

Techniques: